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Urine was also examined for Chlamydia trachomatis and Neisseria gonorrhoeae by polymerase chain reaction (PCR; Amplicor, Roche Diagnostics, Branchburg, New Jersey, USA). Sera had been examined for HIV-1 and HIV-2 using the Murex HIV Ag/Ab Combination ELISA (Murex Biotech). Urine specimens from feminine contributors have been tested for pregnancy using an IPAS dipstick (Quickstick One Step HCG Pregnancy Test; IPAS/Pharmatech Inc., Denver, Colorado, USA). Lifetime exposure to syphilis was examined utilizing a Treponema pallidum particle agglutination (TPPA) check (Serodia TPPA; Fujirebio Inc., Tokyo, Japan).

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